Activity 9 Genetic Analysis The corn you will analyze was produced by crossing two true-breeding (homozygous) individuals (the P generation), one purple and one yellow, to produce the F1 generation. Two individuals from the F1 then served as parents to produce the F2 generation. Each of the kernels you will count is a single member of the F2 generation.
For the following corn, score at 4 rows for color (purple or yellow). Record your results in the table below.
You are going to estimate how far your observed values are from your expected values by calculating a χ 2 (chi square). The smaller your χ2, the smaller the deviations. You will work with one decimal place. Show all your work!
**Expected: you will calculate this numbers by multiplying the total number of kernels counted by the expected phenotypic frequency of each class. The expected phenotypic frequencies are the ones you estimated above. If you did this correctly, the observed and expected totals should be the same.
Determine the degrees of freedom (df).The degrees of freedom are calculated as the one less than the number of categories (in this case, flavors). (df = n-1). Record your df below.
In 1978, Yuet Wai Kan and Andrees Dozy of the University of California-San Francisco showed that the restriction enzyme Mst II, which cuts normal hemoglobin DNA at a particular site, will not recognize (and therefore will not cut) DNA that contains the sickle cell mutation. Thus, the DNA from normal homozygous individuals (AA), heterozygous carriers of the trait (AS), and homozygous sickle cell patients (SS) produces different sizes of restriction fragments when cut with.
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